Figure 6.

BIGE1-GFP Is Localized to the trans-Golgi.

Subcellular localization of BIGE1-GFP in roots of Arabidopsis plants transformed with Pro35S:BIGE1-GFP was determined using electron microscopy immunocytology.

(A) Confocal image of GFP fluorescence in root cells of a transgenic plant. Red line outlines a single root cell in the elongation zone.

(B) Localization of the BIGE1-GFP fusion protein detected by gold-labeled GFP polyclonal antibody.

(C) Schematic illustration of the image in (B) showing the position of the Golgi apparatus (blue). Trans and Cis indicate polarity of the Golgi apparatus. Immunolocalization images of two independent samples and a Col wild-type control sample are shown in Supplemental Figure 6.