Figure 7.

RT-qPCR Analysis of CYP78A Genes in bige1 Mutant Embryos.

Expression of PLA1A, GE1, and GE2 genes in developing embryos of normal (WT) and the bige1-umu1 and bige1-umu5 mutants harvested from segregating ears grown in our 2013 spring field. Values were normalized to 18S rRNA, with expression in wild-type 12-DAP embryos set to 1. The values are means of technical duplicate assays. White circles represent the wild type, and black circles represent bige1 mutant. Independent replicate experiments are shown in Supplemental Figure 8.