Figure 6.

LUH and PIF1 Bind an Overlapping Subset of Genes in Imbibed Seeds.

Enrichment of 18 PIF1-regulated target promoters by PIF1 (A) and LUH (B). ChIP assays were performed with an anti-Myc antibody using imbibed phyB_off GFP-OX (GFP), LUH-OX (LUH), and PIF1-OX (PIF1) seeds. The enrichment of each promoter was normalized both by the level of input DNA and by the ChIP enrichment value of the nonbinding PP2A promoter (sd, n = 3 biological replicates). Genes whose expression was either up- or downregulated in both luh and pif1 mutant seeds are indicated.