Table 2. Predicted Duplexes Formed between the Tomato CalS12-like mRNA Target and vd-sRNAs Derived from the VMRs of PSTVd Variants.

PSTVd Variant	CalS12-like mRNA/vd-sRNA	∆Ga	% Pairing
PSTVd-I	3670 GG A G A A 3690	−22.06	71.4
	5′-U UUC U UC GCUCAGGA -3′
	3′-A AAG A AG CGAGUCCU -5′
	59 AA A A A C 39
PSTVd-I	3668 GG A G A 3689	−22.95	77.3
	5′-CUU UUC U UC GCUCAGGA-3′
	3′-GAA AAG A AG CGAGUCCU-5′
	61 AA A A A 40
PSTVd-M	3670 GG A G A A A 3690	−16.21	66.7
	5′-U UUC U UC GCTC GGA -3′
	3′-A AAG A AG CGAG UCU -5′
	59 AA A A A C C 39
PSTVd-M	3668 GG AG A A 3689	−17.1	68.2
	5′-CUU UUC U UC GCUC GGA-3′
	3′-GAA AAG A AG CGAG UCU-5′
	61 AA A A A C 40

^aThe PairFold online tool was used to predict the minimum secondary structure free energy of pairs of RNA sequences.

Underlined nucleotides represent G:U wobble base pairs.