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. Author manuscript; available in PMC: 2016 Mar 10.
Published in final edited form as: Nature. 2015 Sep 2;525(7568):206–211. doi: 10.1038/nature15251

Extended Data Figure 8. MLL knockdown reduces proliferation specifically of GOF p53 MEFs and LFS cells.

Extended Data Figure 8

a. Growth curve analysis of GOF p53 R172H MEFs with either non-targeting control shRNA or two different mll1 shRNA knockdown.

b. Western blot analysis of MLL1 levels upon shRNA-mediated knockdown in LFS MDAH087 and MDAH041 cells.

c. Western blot analysis of p53 protein levels in LFS MDAH087 and MDAH041 cells.

d, e. Growth curve analysis of LFS MDAH087 cells upon (d) mll1 knockdown or (e) p53 knockdown.

f. Growth curve analysis of LFS MDAH041 cells upon mll1 knockdown.

g, h. (g) Western blot analysis of MLL1 level and (h) growth curve analysis of proliferation upon shRNA-mediated mll1 knockdown in IMR90 cells.

i. Growth curve analysis of LFS MDAH087 cells with non-targeting control shRNA plus empty vector, p53 shRNA plus vector, and p53 shRNA plus MLL1 expressing vector.

j, k. Growth curve analysis of (j) LFS MDAH087 and (k) LFS MDAH041 cells with either non-targeting control shRNA or mll2 shRNA knockdown.