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. Author manuscript; available in PMC: 2016 Mar 10.
Published in final edited form as: Nature. 2015 Sep 2;525(7568):206–211. doi: 10.1038/nature15251

Figure 2. GOF p53 mutants directly target chromatin regulators.

Figure 2

a. Track views of p53 occupancy over promoter regions of mll1, mll2, moz and p21.

b, c. ChIP-qPCR showing p53 or IgG enrichment (ChIP/Input) in (b) MDA-MB-468 and (c) MDA-MB-175VII cells. BS: p53 binding site. Primer locations shown in Extended Data Fig. 4a.

d, e. ChIP-qPCR showing p53 enrichment changes upon (d) p53 or (e) ets2 knockdown. #20 and #21 denote two short hairpins, sequences of which are shown in Table S3.

f. ChIP-qPCR showing p53 or IgG enrichment in MEFs bearing p53 WT or R172H. (Error bars represent mean ± s.e.m., n=3. Two-tailed Student's t-test: *p<0.05; **p<0.01; ***p<0.001)