Table 2. Mutagenesis efficiency of knocking out genes Abdominal-B, ebony and frizzled in P. xuthus.
| Gene | Target sites* | RNA con. (ng μl−1)† | Phynotype rate (%)‡ | Mutation rate (%) | Mutation type |
|---|---|---|---|---|---|
| Px_03961_Abd-B | T42 | 200; 300 | 7.95 (7/88) | 35.38 | D: 3–51; I: 4–18 |
| 600; 600 | 8.26 (10/121) | 25 | D: 3–66; I: 4–5; M: 40 | ||
| T42/T95 | 50/50; 100 | 6 (6/100) | 18.33 | D: 5–59; I: 3–19 | |
| 566/416; 1,200 | 91.79 (123/134) | 90.85 | D: 3–73; I: 3–64; M: 3–29 | ||
| Px_01073_e | T2/T303 | 158/159; 1,200 | 31.25 (5/16) | 66.67 | D: 5–33; I: 3–10; M: 3 |
| T454/T6 | 200/150; 1200 | 88 (22/25) | 29.92 | D: 6–62; I: 3–15; M: 3–8 | |
| Px_15230_fz | T268/T283 | 500/500; 1,,000 | 4.12 (4/96) | 45.16 | D: 3–64; I: 3–15; M: 4–5 |
D, delete fragment (bp); I, insert fragment (bp); M, mutated fragment (bp); sgRNA, single guide RNA.
*‘/' denotes injection of mixed targets.
†The number before and after semicolon denotes injected concentration of sgRNA and Cas9 mRNA, respectively.
‡The number in the bracket denotes mutant/observed injected individuals.