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. 2015 Sep 10;6:8212. doi: 10.1038/ncomms9212

Table 2. Mutagenesis efficiency of knocking out genes Abdominal-B, ebony and frizzled in P. xuthus.

Gene Target sites* RNA con. (ngμl−1) Phynotype rate (%) Mutation rate (%) Mutation type
Px_03961_Abd-B T42 200; 300 7.95 (7/88) 35.38 D: 3–51; I: 4–18
    600; 600 8.26 (10/121) 25 D: 3–66; I: 4–5; M: 40
  T42/T95 50/50; 100 6 (6/100) 18.33 D: 5–59; I: 3–19
    566/416; 1,200 91.79 (123/134) 90.85 D: 3–73; I: 3–64; M: 3–29
Px_01073_e T2/T303 158/159; 1,200 31.25 (5/16) 66.67 D: 5–33; I: 3–10; M: 3
  T454/T6 200/150; 1200 88 (22/25) 29.92 D: 6–62; I: 3–15; M: 3–8
Px_15230_fz T268/T283 500/500; 1,,000 4.12 (4/96) 45.16 D: 3–64; I: 3–15; M: 4–5

D, delete fragment (bp); I, insert fragment (bp); M, mutated fragment (bp); sgRNA, single guide RNA.

*‘/' denotes injection of mixed targets.

The number before and after semicolon denotes injected concentration of sgRNA and Cas9 mRNA, respectively.

The number in the bracket denotes mutant/observed injected individuals.