Table 3.
Divergence analysis for GGT subfamilies: Divergence type I and type II analyses of two clusters comparisons are shown here in details
| Bacteria 1/Bacteria 4-Extremophile | Bacteria 1/Eukaryote | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| FD I | FD II | FD I | FD II | ||||||||
| θ I = 0.49 ± 0.08 | θ II =0.31 ± 0.09 | θ I = 0.58 ± 0.12 | θ II = 0.31 ± 0.08 | ||||||||
| P = 0.75 | R = 9.50 | P = 0.70 | R = 10.35 | ||||||||
| Site | E c | B h | Site | E c | B h | Site | E c | Hm | Site | E c | Hm |
| G 1 | G 1 | G 1 | G 1 | G 1 | G 1 | G 1 | G 1 | G 1 | G 1 | G 1 | G 1 |
| 415 | P291 | P268 | 215 | R114 | S88 | 544 | V396 | V386 | 540 | T392 | A382 |
| 463 | A335 | V309 | 540 | T392 | V359 | 546 | D398 | A388 | 631 | S481 | A471 |
| 465 | R337 | G311 | 541 | H393 | Y360 | 629 | T479 | V367 | 638 | I488 | T478 |
| 474 | F346 | D320 | 555 | T407 | I374 | 632 | P482 | A472 | |||
| 478 | D350 | S319 | 559 | N411 | Y378 | 641 | V491 | T481 | |||
| 535 | E387 | P354 | 611 | S462 | I422 | 725 | I556 | I547 | |||
| 576 | N428 | Q395 | 631 | S481 | V438 | 758 | E563 | G553 | |||
| 583 | S435 | S402 | 632 | P482 | M439 | ||||||
| 602 | A453 | A410 | 637 | I487 | Q444 | ||||||
| 638 | I488 | P445 | |||||||||
| G 2 | G 2 | G 2 | G 2 | G 2 | G 2 | G 2 | G 2 | G 2 | G 2 | G 2 | G 2 |
| 264 | Y161 | Y133 | 184 | A84 | T59 | 163 | I65 | A57 | 184 | A84 | G76 |
| 275 | V170 | L142 | 187 | H87 | T64 | 287 | I182 | P169 | 187 | H87 | N79 |
| 276 | Q171 | A143 | 190 | A90 | N66 | 342 | L231 | Y217 | 188 | P88 | A80 |
| 287 | I182 | P154 | 196 | G95 | D71 | 383 | A269 | N255 | 190 | A90 | S82 |
| 349 | G238 | N215 | 213 | D112 | N86 | 409 | Y285 | V272 | 191 | G91 | M83 |
| 351 | D240 | E217 | 216 | E115 | G89 | 491 | K361 | A352 | 197 | G97 | L89 |
| 398 | R283 | R260 | 338 | L227 | A205 | 647 | N497 | Y487 | 213 | D112 | N105 |
| 409 | Y285 | Y262 | 389 | R275 | V252 | 687 | D532 | A522 | 249 | G147 | A133 |
| 432 | E308 | K284 | 543 | S395 | A362 | 719 | G551 | A542 | 261 | L158 | H145 |
| 440 | G316 | D292 | 579 | M431 | G398 | 264 | Y161 | H148 | |||
| 486 | N356 | S330 | 641 | S465 | H419 | 266 | T163 | R150 | |||
| 487 | K357 | D331 | 665 | H515 | Q472 | 278 | A173 | S160 | |||
| 489 | Y359 | Y333 | 316 | K206 | C192 | ||||||
| 545 | V397 | A364 | 362 | A248 | T234 | ||||||
| 550 | N402 | N369 | 389 | R275 | I261 | ||||||
| 624 | K474 | Q431 | 395 | G280 | I267 | ||||||
| 649 | I499 | I456 | 398 | R283 | G270 | ||||||
| 675 | D521 | K477 | 408 | G284 | D271 | ||||||
| 687 | D532 | D488 | 414 | M290 | P277 | ||||||
| 759 | L564 | L524 | 418 | P292 | A279 | ||||||
| 416 | S294 | L281 | |||||||||
| 423 | H299 | V285 | |||||||||
| 432 | E308 | K294 | |||||||||
| 464 | D336 | K326 | |||||||||
| 487 | K357 | E348 | |||||||||
| 585 | K437 | P427 | |||||||||
| 608 | R459 | Q448 | |||||||||
| 643 | Q493 | L483 | |||||||||
| 650 | D500 | W490 | |||||||||
| 675 | D521 | N511 | |||||||||
| 761 | G566 | A556 | |||||||||
θI and θII >0 are observed for type I and type II analysis respectively in each case. Corresponding residues are marked on the two representative proteins of the compared clusters with respect to predicted divergence sites by FDI and FDII methods. Divergence sites predicted through the analysis are divided into two groups, G1 and G2, where G1 represents the sites that are close to the known functional residues forming the substrate binding cavity and G2 represents all other sites. Ec: gamma glutamyl transpeptidase of Escherichia coli. Bh: Gamma glutamyl transpeptidase of Bacillus halodurans. Hm: gamma glutamyl transpeptidase of human