Fig. S7.

Respiration analysis of primary hepatocytes determined by Seahorse XF96 Extracellular Flux Analyzer. Hepatocytes were isolated from livers 4 d after treatment with CCl₄ and cultured overnight. After baseline OCR determination, cells were sequentially treated with oligomycin A (1 µM), FCCP (0.3 µM), and rotenone (1 µM) + antimycin A (1 µM) as indicated by the arrows. The basal respiration rate of the KO hepatocytes was dramatically decreased compared with the WT cells. Top1mt KO hepatocytes were friable, and oxygen consumption could not stabilize after FCCP treatment.