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Generation of TOP1mt KO cells using CRISPR/Cas9. (A) Western blot of whole-cell lysate using Top1mt-specific antibody (5). ATP5A was included as loading control. (B) Relative mRNA expression levels of Top1mt in HCT116 parental cells and Top1mt KO cells (clone 1, clone 2). (C) Representative gel of the PCR product of Top1mt following qPCR.
