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Symbionts released into the water of high-pressure vessels and filtered. Endoriftia symbionts detected in the water column of the high-pressure vessel after 8 d of incubation under simulated deep-sea conditions without flow. Red, EUB338 probe mix; blue, DAPI as a general nucleic acid stain for symbiont nuclei; green, symbiont-specific probe RifTO445. (Scale bar, 5 µm.)
