Table S1.
Antibodies and staining protocols used in this study
| Primary antibody | Source/vendor | Protocol | Cell types marked (reference) |
| Rat α-BrdU | Abcam | Pre-Tx: 6N HCl for 10 min or steam for 10 min (1:200) → bDαRat → fluor-SA | Dividing cells labeled with a BrdU pulse |
| Rb α-CK14 | Labvision | (1:500) → fluor-DαRb | HBCs (21) |
| Gt α-mouse CD54 | R&D | (1:100) → fluor-DαGt or (1:400) → bDαGt → fluor-SA | HBCs (58) |
| Rb α-dsRed | Rockland Inc. | (1:400) → fluor-DαRb | All TdTomato+ cells |
| Rb α-Ki67 | Epitomics | (1:100) → fluor- DαRb | Cells in the cell cycle (33, 59) |
| Gt α-mCherry | Acris | (1:150) →fluor-DαGt | All TdTomato+ cells |
| Gt α-OMP | Wako (Frank Margolis, University of Maryland, Baltimore) | (1:400) → DAB or (1:100) → fluor-DαGt | Mature OSNs (34) |
| Mo α-p63 | Santa Cruz | Pre-Tx: Steam (1:150) → bDαMo →fluor-SA |
All HBCs, except early activated HBCs (20) |
| Rb α-pH3(Ser10) | Millipore | (1:300) →fluor-DαRb | Cells in mitosis (59) |
| Ch α-PGP9.5 | Rockland Inc. | Pre-Tx: 6N HCl for 10 min (1:200) → fluor-DαCh | All neurons (29) |
| Rb α-PGP9.5 | Ultraclone | (1:1,200) → fluor-DαRb or (1:5,000) → DAB | All neurons (29) |
| Gt α-Sox2 | Santa Cruz | Pre-Tx: DNaseI + Steam or 30 mins 65 °C (1:25) → bDαGt → fluor-SA or DAB | Sus cells, HBCs, GBCs (17) |
| Rb α-Sox9 | Millipore | Pre-Tx: Steam+EtOH (1:300) → bDαGt → fluor-SA | Duct/gland, microvillar cells (60) |
| GP α-TrpM5 | Gift from E. Liman (University of Southern California, Los Angeles) | (1:400) → DAB | microvillar cells (61) |
Antibody hosts: Ch, chicken; D, donkey; GP, guinea pig; Gt, goat; Mo, mouse; Rb, rabbit. Antibody visualization: DAB: the HRP substrate diaminobenzidine was used for chromogenic staining; DNaseI: sections were treated with 500 U/mL DNaseI in buffer (40 mM Tris⋅HCl, 10 mM NaCl, 6 mM MgCl2, 10 mM CaCl2, pH 7.9) for 10 min; EtOH: slides were dehydrated and rehydrated through graded EtOH (70% →95% →100% →95% → 70%), 1 min each; fluor: a variety of Alexa fluorophores, including Alexa 405, 488, 546, 594, and 647(Jackson Immunological) were used at 1:100; tertiary reagent amplification: biotinylated secondary antibody (bDαX) was followed by incubation in fluor-streptavidin (fluor-SA). Pretreatments (Pretx): 30 min 65 °C: slides were incubated in 0.01 M citrate, pH6, at 65 °C for 30 min; 6N HCl: sections were preincubated in 6N HCl for 10 min; steam: sections wee covered with 0.01 M citrate, pH6, and steamed in a commercial food steamer for 10 min.