Migration analysis of Nrp2 knockout mice. Immunohistochemical staining for Nrp2-tauGFP (green), calbindin (red), and COUP-TFII (blue) in an E13.5 horizontal section of a heterozygous (+/−) or homozygous (−/−) Nrp2-Δ mouse brain. No significant difference in migration was observed. Ctx, cortex; GE, ganglionic eminence. (Scale bars, 200 µm.)