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. 2015 Aug 24;112(36):11324–11329. doi: 10.1073/pnas.1509968112

Fig. 2.

Fig. 2.

USP25 is required for HSV-1–induced production of type I IFNs and proinflammatory cytokines. (A) HSV-1–induced expression of Ifnb and Il6 was inhibited in Usp25−/− cells. Wild-type or Usp25−/− MLFs, BMDCs, and FLT3L-pDCs were infected with HSV-1 or treated with CpG for the indicated time points before qPCR analysis was performed. (B) Usp25−/− cells produced reduced levels of IFN-α and IL-6 cytokines after HSV-1 infection compared with the wild-type counterparts. Wild-type or Usp25−/− BMDMs, BMDCs, FLT3L-pDCs were infected with HSV-1 or treated with CpG for 12 h and the supernatants were collected for ELISA analysis. (C) HSV-1 infection-induced phosphorylation of IRF3 and IκBα was impaired in Usp25−/− BMDCs compared with the wild-type BMDCs. Wild-type or Usp25−/− BMDCs were infected with HSV-1 for 6–12 h. Cells were lysed and cell lysates were subject to immunoblot with the indicated antibodies. Data are representative of three independent experiments (mean and SD of three replicates in A and B).