Fig. S2.

USP25 positively regulates RNA virus-induced signaling in primary mouse lung fibroblasts (MLFs). (A) The expression of Ifnb, Ifna4, Tnf, and/or Il6 was inhibited in Usp25^−/− MLFs than in wild-type counterparts after SeV or VSV infection or transfection of the RNA analog poly(I:C). (B) Usp25^−/− MLFs produced decreased amount of IFN-α, IL-6 and TNF-α proteins after SeV or VSV infection than did the wild-type cells. (C) SeV- or VSV-induced phosphorylation of IRF3 and IκBα was substantially impaired in Usp25^−/− MLFs. Data are representative of two independent experiments (means and SD of three replicates in A and B).