Fig. S5.

Usp25^−/− mice produced decreased amounts of IFN-α and IL-6 cytokines and exhibited less infiltration of monocytes after VSV infection than did wild-type mice. (A) Usp25^−/− mice produced significantly decreased amounts of IFN-α and IL-6 cytokines than did wild-type mice. Wild-type and Usp25^−/− mice were infected with VSV (2 × 10⁷) through tail vein. Twelve hours after infection, the sera were collected and the amount of IFN-α or IL-6 in the sera was determined by ELISA analysis. (B) The infiltration of monocytes in the lungs of Usp25^−/− mice was impaired compared with that of wild-type mice. Lung tissues from mice treated in A were isolated and subject to histological analysis with hematoxylin and eosin staining (H&E) 24 h after infection. [Original magnification, ×20 (H&E) or ×40 (Ly6C).] (Scale bars, 100 μm.) Data are representative of two independent experiments. *P < 0.05, **P < 0.01.