Fig. S6.

USP25 regulates the stability of TRAF3 and TRAF6 proteins. (A) Wild-type or Usp25^−/− MEFs were left untreated or treated with CHX (100 μg/mL) for one hour followed by SeV or HSV-1 infection. Cells were lyzed and the immunoblot analysis was performed at 8 h after infection. (B and C) Wild-type and Usp25^−/− BMDCs or MLFs were infected with SeV or HSV-1 for the indicated time points before qPCR analysis was performed. Data are representative of two (A) or at least three (B and C) independent experiments (graphs show mean and SD of three replicates).