Fig. S9.

USP25 stabilizes TRAF6 through proteasome-independent pathways after virus infection. (A) USP25 deficiency resulted in accelerated degradation of TRAF6 without altering the K48-linked ubiquitination of TRAF6 after viral infection. Wild-type and Usp25^−/− BMDCs were infected with SeV or HSV-1 for the indicated time points followed by denature-IP and immunoblot assays. (B) SeV-induced degradation of TRAF6 in Usp25^−/− MEFs was inhibited by the autophagy inhibitor 3MA. Wild-type and Usp25^−/− MEFs were pretreated with MG132 (10 nM) or 3MA (0.5 μg/mL) for 2 h and infected with SeV for 8 h before immunoblot analysis was performed with the indicated antibodies. Data are representative of two independent experiments.