Fig. S10.

Reconstitution of MAVS into Usp25^−/− MEFs partially restored SeV-triggered expression of type I IFNs and proinflammatory cytokines. (A) Wild-type or Usp25^−/− MEFs were transfected with Phage-puro (Vec) or Phage-puro-Flag-MAVS followed by selection with puromycin (1 μg/mL) for 2 d. The puromycin-resistant cells were subject to immunoblot with the indicated antibodies. (B) The expression of type I IFNs and proinflammatory cytokines in cells obtained from A. Cells were infected with SeV or left uninfected for 12 h before qPCR analysis was performed. Data are representative of two independent experiments.