Table S1.
Strains used in this study
| Strain | Genotype or relevant features | Source or reference |
| B. subtilis | ||
| PY79 | Wild-type | C. Ellermeier, The University of Iowa |
| E. coli strains for experiments | ||
| EC251 | MG1655 (wild-type) | Lab collection |
| EC1898 | EC251/pDSW997 | (17) |
| EC2368 | EC251/pDSW962 | (50) |
| EC2691 | RP77/pDSW997 | This study |
| EC2723 | RP77/pDSW962 | This study |
| EC3486 | EC251 ΔamiA<>frt ΔamiC<>frt | This study |
| EC3708 | ΔrlpA<>frt ΔmltA<>frt Δslt<>frt ΔmltD<>frt ∆mltE<>frt ∆mltC<>frt | This study |
| EC3906 | EC3486/pDSW997 | This study |
| EC3907 | EC3486/pDSW962 | This study |
| EC3908 | EC3708/pDSW997 | This study |
| EC3909 | EC3708/pDSW962 | This study |
| RP77 | CS109 amiB yje::res amiA::cam amiC::kan | (10) |
| E. coli donors for strain construction by P1 transduction | ||
| JW2428-1 | BW25113 ΔamiA764::kan | (51) |
| JW2542-6 | BW25113 ΔmltF732::kan | (51) |
| JW2671-1 | BW25113 ΔmltB776::kan | (51) |
| JW2784-1 | BW25113 ΔmltA741::kan | (51) |
| JW5018-1 | BW25113 ΔmltD730::kan | (51) |
| JW5449-1 | BW25113 ΔamiC742::kan | (51) |
| JW5481-1 | BW25113 ΔmltC738::kan | (51) |
| JW5821-1 | BW25113 ΔemtA783::kan (emtA is also called mltE) | (51) |
| E. coli plasmid hosts for cloning or protein overproduction | ||
| BL21 | fhuA2 [lon] ompT gal [dcm] ΔhsdS | New England Biolabs |
| BL21(DE3) | λ(DE3) fhuA2 [lon] ompT gal [dcm] ΔhsdS | (28) |
| Shuffle T7 | F′ lac, pro, lacIQ/Δ(ara-leu)7697 araD139 fhuA2 lacZ::T7 gene1 Δ(phoA)PvuII phoR ahpC* galE (or U) galK λatt::pNEB3-r1-cDsbC (SpecR, lacIq) ΔtrxB rpsL150(StrR) Δgor Δ(malF)3 | New England Biolabs |
| XL1-Blue | F′ proAB lacIq lacZΔM15 Tn10 Tetr/recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac | Stratagene |
| Protein overproduction strains | ||
| EC1903 | BL21/pDSW1000 (for His6-DamXSPOR) | (17) |
| EC2068 | BL21/pDSW1086 (for His6-DamXSPOR(Q351K)) | (21) |
| EC2249 | BL21(DE3)/pDSW1171 (for His6-GFP-DamXSPOR) | This study |
| EC2251 | BL21(DE3)/pDSW1171 (for His6-GFP-DedDSPOR) | This study |
| EC2292 | BL21(DE3)/pET-28a-amiD (for His6-AmiD) | (28) |
| EC3087 | BL21/pDSW1557 (for His6-PaRlpA) | (27) |
| EC3204 | BL21/pDSW1600 (for His6-PaRlpA(D157N)) | (27) |
| EC3387 | BL21/pDSW1650 (for His6-PaRlpA(ΔSPOR)-mCherry) | This study |
| EC3476 | Shuffle T7/pDSW1666 (for His6-GFP-FtsNSPOR) | This study |
| EC3477 | BL21/pDSW1667 (for His6-GFP-RlpASPOR) | This study |
| EC3479 | BL21/pDSW1669 (for His6-GFP-DamXSPOR(Q351K)) | This study |