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. 2015 Aug 24;112(36):11252–11257. doi: 10.1073/pnas.1512197112

Fig. S1.

Fig. S1.

Mad2ΔN exclusively forms the asymmetric I-Mad2–C-Mad2 dimer in the absence of ligands. (A) Overlay of the methyl region of the 1H/13C HSQC spectra of 13C-labeled O-Mad2ΔC (black) and 13C-labeled I-Mad2ΔC bound to unlabeled C-Mad2–MBP1 complex (red). (B) Overlay of the methyl region of the 1H/13C HSQC spectra of 13C-labeled C-Mad2 bound to unlabeled MBP1 (black) and 13C-labeled I-Mad2ΔC bound to unlabeled C-Mad2–MBP1 complex (red). (C) Overlay of the methyl region of the 1H/13C HSQC spectra of 13C-labeled Mad2ΔN dimer (I-Mad2–C-Mad2) (magenta) and 13C-labeled I-Mad2ΔC–C-Mad2 (bound to unlabeled MBP1) (black).