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Short telomeres are not sufficient to cause reduced Pot1 binding. (Upper Left) ChIP assay demonstrating Pot1 relative fold enrichment at wild-type (30 °C) and short (37 °C) telomeres. ChIP was performed as described in Fig. 4. (Bottom Left) Western blot showing levels of Pot1-Myc in input lysates and immunoprecipitates. (Right) Telomere blot comparing telomere lengths of pot1-Myc wild-type cells before and after growth at 37 °C to shorten telomeres.
