Fig. S3.

Sld3 stimulation of DDK phosphorylation of Mcm2 is required for RPA-CHiP signal at an origin. Chromatin immunoprecipitation was performed using cells described in Fig. 2A, Right at the restrictive temperature, in the presence of galactose, and in a wild-type background. Cells were arrested with α-factor (G₁ cells) and then released for 20 min (S phase cells). Cells extracts were fixed and immunoprecipitated with antibodies directed against RPA. The immunoprecipitate was probed for DNA sequence using quantitative PCR at two early origins (ARS305 or ARS306) and at a region midway between these origins. Results from repeated experiments were quantified and plotted.