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. 2015 Jul 15;5(8):2396–2408.

Figure 4.

Figure 4

As+Cd+Pb up-regulates dose-dependent IL-1 and IL-1R1, inducing cleaved caspase-9 expression in C6. Eighty percent confluent C6 cells were incubated with vehicle (V, water) or As+Cd+Pb at the three different concentrations overnight, and IL-1, IL-1R1 and cleaved caspase-9 expressions were analyzed. A. Representative IL-1 immunostaining (red fluorescence). Hoechst staining: blue fluorescence. B. Representative IL-1R1 immunostaining (red fluorescence). Hoechst staining: blue fluorescence. C. Representative IL-1 (red fluorescence) and GFAP (green fluorescence) co-immunostaining. Hoechst staining: blue fluorescence. D. Representative IL-1R1 (red fluorescence) and GFAP (green fluorescence) co-immunostaining. Hoechst staining: blue fluorescence. E. Representative cleaved caspase-9 immunostaining (red fluorescence) upon co-treatment with IL-1Ra. Hoechst staining: blue fluorescence.