Figure 4. Effect of intracerebroventricular administration of specific NOS inhibitors on the antinociception induced by acute aerobic exercise (AE) in the paw-withdrawal (A) and tail-flick (B) tests. Rats were pretreated with intracerebroventricular injection of aminoguanidine (AMG, 10 μg/5 µL), N 5-(1-iminoethyl)-L-ornithine dihydrochloride (L-NIO, 10 μg/5 µL) and Nω-propyl-L-arginine (L-NPA, 10 μg/5 µL) immediately before the onset of exercise, which lasted for a mean of 43.3±1.0 min. Mechanical and thermal nociceptive thresholds were measured before and after 1, 15, 30 min of AE. Data are reported as means±SE of 6 animals per group. ***P<0.001, compared to the control group (Co); ###P<0.001, compared to the AE group (one-way ANOVA followed by the Bonferroni test). Vec: vehicle; BL: baseline latency.
