Table 1. Detection of IgM and IgG antibodies to B. burgdorferi in the examined groups of forestry workers, soldiers and healthy blood donors by serological examinations using two-step protocol, ELISA test and confirmatory Western blot method.
Results of serological examinations | IgM + IgG + | IgM + IgG − | IgM +/− IgG + | IgM +/− IgG +/− | IgM + IgG +/− | IgM +/− IgG − | IgM − IgG + | IgM − IgG +/− | IgM − IgG − | Total number of samples | The obtained seroprevalence (%) | |
---|---|---|---|---|---|---|---|---|---|---|---|---|
I -group (forestry workers) | ||||||||||||
1. | ELISA | - | - | - | - | - | - | 4 | - | 30 | 34 | |
2. | Western blot | - | - | - | - | - | - | 4 | - | 30 | 34 | |
11.76% | ||||||||||||
II-group (soldiers) | ||||||||||||
1. | ELISA | 1 | 2 | - | - | 1 | 1 | 2 | 1 | 29 | 35 | |
2. | Western blot | 1 | 1 | 1 | 3 | 29 | 35 | |||||
17.14% | ||||||||||||
III-group (healthy blood donors) | ||||||||||||
1. | ELISA | 1 | 2 | 32 | 35 | |||||||
2. | Western blot | 1 | 2 | 32 | 35 | |||||||
8.57% |
Interpretation of the results: + positive result, − negative result, +/− borderline (equivocal) result-“grey zone“.
Seroprevalence was determined in relation to Western blot positivity.