Table 2. Transformation frequency of different lactobacilli.
Voltage (kV) | Transformation frequencya (cfu/μg plasmid DNA) | |
---|---|---|
|
||
Mason procedure | Modified procedure | |
1.5 | 1.1 × 102 | 4.3 × 103 |
2.0 | 2.7 × 102 | 8.6 × 103 |
2.5 | Not detectedb | 3.4 × 103 |
Data are obtained from three experiments.
Less than 10 transformants per μg plasmid DNA.