Table 2. The effect of inhibitors and activators on keratinase and protease activity in the crude culture fluid of M. luteus B1pz.
Inhibitor / activator | Concentration [mM] | Residual activity [%] | |
---|---|---|---|
Keratinolytic activity | PMSF | 10 | 28 |
EDTA | 5 | 100 | |
10 | 30 | ||
NEM | 10 | 0 | |
Cys | 1 | 164 | |
Ca2+ | 5 | 1606 | |
Proteolytic activity | PMSF | 10 | 0 |
EDTA | 5 | 100 | |
10 | 100 | ||
NEM | 5 | 86 | |
10 | 23 | ||
Cys | 1 | 586 | |
Ca2+ | 10 | 814 |