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| Variable | Code | Level of variation | ||||
|---|---|---|---|---|---|---|
|
|
||||||
| −1.68 | −1 | 0 | 1 | 1.68 | ||
| JAP (g/L) | A | 53.2 | 60.0 | 70.0 | 80.0 | 86.8 |
| Peptone (g/L) | B | 9.9 | 15.0 | 22.5 | 30.0 | 35.1 |
| Solution volume (mL) | C | 33.2 | 40.0 | 50.0 | 60.0 | 66.8 |
