Figure 4.

Cardiac hypertrophy and fibrosis in methyl-CpG–binding protein 2-transgenic (MeCP2-TG) mice. A and B, Hematoxylin-eosin (top) and wheat germ agglutinin stained (lower panels) cardiac sections of sham (control) and transverse aortic constriction (TAC)–operated mice. Ventricular weight/body weight ratios (n=4–15 per group; C) and cardiac myocyte cross-sectional areas (n=4–9 per group; D). E–G, Interstitial cardiac fibrosis identified by Sirius red staining and morphometric analysis of fibrotic areas (G; n=4–9 per group). H, TUNEL (TdT-mediated dUTP-biotin nick end labeling) staining to detect apoptotic cells (n=5–10 per group). Mean±SEM; *P<0.05; **P<0.01; ***P<0.001. LV indicates left ventricle.