Figure 2.

AChE activity in the exosomal fraction. The exosomes were isolated from the heart and serum of the exercise (db/db and control) and non-exercise (db/db and control) groups by ultracentrifugation. AChE activity was assessed in the exosomal fraction by Ellman assay 26. Different concentrations of exosomes were used to standardize the assay. The exosomes suspended in PBS were diluted in AChE working solution and incubated at 37°C in dark for 30 min. OD was measured at 412 nm at regular time intervals of 5 min. The exosomal fraction from the exercise group had significantly higher activity as compared to the non-exercise group (*P < 0.05).