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. 2015 Mar 30;19(9):2153–2161. doi: 10.1111/jcmm.12589

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© 2015 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Confirmation of exosomal markers CD81 and flottilin 1. The exosomal fraction from both heart and serum was separated on SDS PAGE (A) and transferred to hybond N⁺ for Western blotting. The first lane shows the exosomes from the serum of db/db control mice (Jackson Laboratory) without exercise while the second lane shows the same with exercise. (B) The exosomal fractions showed higher expression of CD81 and flottilin 1 proteins in the exercise group as compared to the non-exercise group.