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. 2015 Jun 8;19(9):2162–2171. doi: 10.1111/jcmm.12595

Figure 5.

Figure 5

Effect of HDAC5 knock down on cytokine production and NF-κB activation. (A) RAW264.7 cells transfected with siRNA against HDAC5 (siHDAC5) or control siRNA (siCtrl) were stimulated with LPS for 16 hrs before concentrations of TNFα, MCP-1, IL-10 or IL-6 were determined from the supernatant. Median values and ranges of n = 4–10 from 3–4 independent experiments. (B) Cells transfected with NF-κB reporter plasmid in combination with siHDAC5 or siCtrl as indicated were incubated for 1 hr in the absence or presence of LPS. NF-κB activation was measured in relative light units. Median values and ranges of n = 7 from 2 independent experiments. (C) U937 cells transfected with siHDAC5 or siCtrl were treated with LPS for 16 hrs before production of TNFα was measured from the supernatant. Median values and ranges of n = 8 from 4 independent experiments. (D) RAW264.7 cells co-transfected with siHDAC5 or siCtrl and pHDAC5 were stimulated with LPS for 16 hrs before TNFα or MCP-1 in the supernatant was determined. Median values and ranges of n = 8 from 3 independent experiments. *P < 0.05, ***P < 0.001 by Mann–Whitney test (A, C) or by Kruskal–Wallis test and Mann–Whitney test as post-test (D).