Figure 4.

BM cells from CD55^−/− CD59^−/− mice have normal adhesion and chemotaxis and do not expand in transplanted wild-type control animals (A). BM Sca-1⁺ cells from CD55^−/− CD59^−/− mice were tested in short (5 min.) and prolonged (15 min.) fibronectin adhesion assays with or without stimulation by SDF-1. Data are pooled from experiments on BM cells isolated from three different mice (B). BM Sca-1⁺ cells from CD55^−/− CD59^−/− mice were tested in a Transwell chemotaxis assay in response to different SDF-1 gradients (50–300 ng/ml). Data are pooled for experiments on BM cells isolated from three different mice. (C). Normal wild-type mice were transplanted with BM from CD55^−/− CD59^−/− mice mixed in a 1:1 ratio with BM from wild-type littermates. These animals maintained ∼1:1 chimerism. Shown are FACS data performed on BM pooled from six recipient mice.