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The expression of NRP1 and the radio-sensitivity of NSCLC cells. (A) The protein level of NRP1 was determined by immunoblotting in U87, PC-3, HepG2, MCF-7, A549 cells. (B) The protein level of NRP1 was determined by immunoblotting in 5 NSCLC cell lines (H358, H460, H1299, A549, SK-MES-1). (C) The cells were exposed to different doses of X-rays, and growth inhibition was examined by MTT assay 24 hrs after irradiation. (D) The change in NRP1 expression was measured after 10 Gy X-rays.
