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. 2015 Jul 14;7(9):1229–1243. doi: 10.15252/emmm.201404669

Figure 6. LXRα enhances glucose uptake and O-GlcNAc signaling via activation of the hexosamine biosynthetic pathway (HBP) in cultured cardiomyocytes.

Figure 6

Neonatal rat ventricular myocytes were transfected with Ad-LXRα or GL2 (Ad-cont), and treatments with phenylephrine (PE) and DON (inhibitor of HBP) were initiated for 24 h.
  • A Assessment of 2-deoxyglucose (2-DG) uptake from 4 independent experiments. *P = 0.03 versus Ad-cont.
  • B, C Glut mRNA expression determined by RT–PCR normalized to 36b4, n = 5 per condition in the absence of PE, n = 4 per condition in the presence of PE. *P = 0.02 versus Ad-cont, **P = 0.008 versus Ad-cont, #P = 0.03, ##P = 0.008.
  • D Western blot indicating Ad-LXRα- and PE-induced increases in global protein O-GlcNAcylation, which was abrogated following inhibition of HBP with DON. LXRα protein expression is shown, and GAPDH served as a loading control.
  • E, F Modulation of Anp and Bnp mRNA levels by Ad-LXRα-induced O-GlcNAc signaling. Gene expression as determined by RT–PCR normalized to 36b4, n = 5 per condition in the absence of PE, n = 4 per condition in the presence of PE. *P = 0.02 versus Ad-cont, **P = 0.008 versus Ad-cont, #P = 0.03, ##P = 0.02.
  • G Measurement of cell size, n = 5 per condition. *P = 0.008 versus Ad-cont, #P = 0.02, ##P = 0.03.
  • H Representative images for the determination of cell size. Cells were stained with an antibody specific for LXRα (green, indicated by arrow), DAPI for nuclei (blue), and rhodamine-phalloidin for F-actin (red); scale bar = 50 μm.

Data information: Data are means ± SEM and are reported as fold change with respect to control group; Kruskal–Wallis test followed by Mann–Whitney U-test was used for group comparisons. Source data are available online for this figure.