Figure 8. O-GlcNAcylation is increased with cardiac LXRα overexpression in mice.
- A, B Western blot analyses of global protein O-GlcNAc levels in left ventricles of mice with either (A) cardiac-specific LXRα overexpression or (B) LXRα deficiency and subjected to 5 weeks TAC.
- C, D Nuclear protein extracts were precipitated with agarose WGA in the absence or presence of GlcNAc, a competitor, and analyzed by Western blot with antibodies against Mef2c, GATA4, or Nkx-2.5, known transcription factors of natriuretic peptides; bands represent 3 pooled hearts per Wt and LXRα-Tg lanes, and (D) quantification is for n = 2, expressed as fold change.
Data information: Data are means ± SEM. IP, immunoprecipitation; WGA, wheat germ agglutinin; GlcNAc, N-acetylglucosamine; WB, Western blot. Source data are available online for this figure.