Table 2.
Challenge Description | True Signal/Activity | False Signal/Assay Interference |
---|---|---|
Nonmonotonic curve: U-shape | ||
Identification of weak signal | N/A | |
Assay interference: cytotoxicity | ||
Assay interference: autofluorescence | ||
Assay interference: contradictory readout | ||
Assay interference: reverse signal |
Ratio or luc, main readout in either β-lactamase assay or luciferase assay, respectively; ch1, channel 1, the background in bla (β-lactamase assay) assay; ch2, channel 2, the signal channel in bla assay; via, cell viability; autofluor, autofluorescence; er, estrogen receptor; ar, androgen receptor; pparg, peroxisome proliferator-activated receptor gamma. Numbers represent different batches.