Table 1.

Post-translational modifications of mitochondrial proteins are less abundant with eicosapentaenoic acid

PTM	Young CON	Old CON	Old EPA	Old DHA
Oxidation	1310.5 ± 21.6	1329.2 ± 13.4	1308.5 ± 6.5	1315.8 ± 13.4
Deamidation	87.3 ± 2.8	90.8 ± 2.7	91.2 ± 2.4	85.0 ± 2.2
Carbamylation	25.3 ± 1.7	29.3 ± 1.2	20.2 ± 2.4*	24.7 ± 1.6
Acetylation	70.5 ± 4.6	82.5 ± 2.9	75.5 ± 3.9	78.8 ± 3.2
Semitryptic Peptides	355.3 ± 17.9	365.8 ± 4.4	345.3 ± 11.1	351.7 ± 5.1

Blind detection of post-translational modifications (PTMs) of mitochondrial proteins including semitryptic peptides was performed using mass spectrometry in muscle tissues (n = 6 per group). To compare PTM abundance across groups, spectral counts were normalized to total mitochondrial proteins and compared using a one-way ANOVA with a Tukey post hoc test. P < 0.05

^*significant difference from old control.