Table 2.

Expression of brachyury protein in primary and metastatic breast carcinoma lesions by immunohistochemistry using a murine monoclonal antibrachyury antibody*

Pt	Tissue	Brachyury
		% Positivity	Intensity
6	Breast primary tumor	30	+
6	Met+ lymph node (a)	90	+
6	Met+ lymph node (b)	90	+
6	Nonmet lymph node (c)	Neg	Neg
9	Breast primary tumor	Focal	+
9	Met+ lymph node (a)	60	++
9	Met+ lymph node (b)	60	++
9	Nonmet lymph node (c)	Neg	Neg
31	Pleura	90	+
32	Bone	90	++
33	Bone	90	+
34	Brain	70	++

* Matched breast primary tumor and metastatic lymph nodes from two patients and metastatic lesions from additional patients were analyzed for brachyury expression by immunohistochemistry with a murine monoclonal anti-brachyury antibody (Abcam). Two lymph nodes positive for metastasis from each patient (a, b) and one lymph node negative for metastasis from the same patient (c) were assayed. Neg = negative; Met = metastatic; Pt = patient number.