Fig 5. Freezing test of acclimated and non-acclimated Col-0, gun5 and cch plants.

Plants were grown for 5 weeks in SD conditions (left panel, non-acclimated) and transferred to SD conditions, 4°C for 3 days (right panel, cold-acclimated). Leaf discs of respective plant were exposed to decreasing temperatures (2°C/h) and checked for electrolyte leakage. Each data point represents values from at least three biological replicates (±SD).