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. 2015 Sep 14;10(9):e0137837. doi: 10.1371/journal.pone.0137837

Fig 10. The effects of the combination of PYC and NF-κB inhibitor (PDTC) on TNF-α and PLIN2 mRNA levels.

Fig 10

Cells were treated with PYC, PDTC (50, 100 μM) or their combination of both for 1h in LPS (500 ng/mL) was then added and further incubated for 24 h. Ribosomal RNAs were used as the total RNA loading control. mRNA level was assessed by real-time PCR, and the mRNA level in the control (no stimuli) was arbitrarily designated as 1 for comparison. The data represent the means ± SD of at least 3 independent experiments. *P< 0.05 compared with LPS alone.