Fig. 3.

E1 and fiber DNA of pure Ad40wt.

Cells were infected with 200 VP/cell of pure Ad40wt. At 72 h pi, encapsidated viral DNA from the the 200 μl supernatants (SNs) and CVLs was purified and Ad40 E1 DNA was tested by PCR on encapsidated viral DNA from the 200 μl CVLs. The results of three independent experiments are shown on the top of figures (a). Plus (+) means reproducible PCR-positive, minus (−) means reproducible PCR-negative, and plus-minus (±) means unreproducible PCR-positive. The Ad40 fiber DNA levels (copy numbers per ml) in the SNs (0 h pi; on the left hand, empty bar graph and 72 h pi; in the middle, shaded bar graph) and CVLs (72 h pi; on the right, black bar graph) from pure Ad40wt were performed by real-time PCR (a). The results shown are mean values of three independent experiments. Error bars indicate the 95% confidence interval. The picture shown is representative of three independent experiments on pure Ad40wt infected 2V6.11 cells (b). The expected DNA size with intact Ad40 E1 is shown on the right of the picture. U, uninduced control (0.1% DMSO). PonA 1 or 5, 1 or 5 μg/ml ponasterone A.