Table 1.
E1b55K mRNA expression and fiber mRNA levels of pure Ad40wt
| Cell lines | Ad40 E1b55K | Ad40 fiber median (IQR) | |
|---|---|---|---|
| 293 | +a | 3.20 (2.62–3.77)b | |
| 2V6.11 |
|
+ | 0.28 (0.01–0.75) |
| + | 0.44 (0.01–0.98) | ||
| + | 0.74 (0.01–1.54) | ||
| 911 | − | 3.92 (2.72–5.11) | |
| 911orf6 |
|
± | 3.86 (2.32–5.39) |
| − | 0.26 (0.01–0.66) | ||
| A549 | − | 1.67 (0.80–2.49) | |
| HeLa | − | 1.77 (0.92–2.61) | |
| W162 | − | 0.01 (0–0.01) |
a
The results of three independent experiments are shown.
b
Pure Ad40wt fiber mRNA levels (% of total RNA) were measured by real-time RT-PCR.
Plus (+) means reproducible PCR-positive, minus (−) means reproducible PCR-negative, and plus-minus (±) means unreproducible PCR-positive.
IQR, interquartile range; U, uninduced control (0.1% DMSO); PonA 1 or 5, 1 or 5 μg/ml PonA.