Table 4. List of amplicons that consistently failed in >10% of samples to reach 250X coverage.

				Samples for which amplicon depth was < 250X
Amplicon_ID	Gene	Exon	GC content (%)	n	%
CHP2_NOTCH1_1	NOTCH1	26	70.9	38	42.7
CHP2_STK11_4	STK11	6	60.4	35	39.3
CHP2_STK11_3	STK11	4–5	75.6	29	32.6
CHP2_FGFR3_2	FGFR3	9	67.9	21	23.6
CHP2_FGFR3_1	FGFR3	7	73.5	16	18.0
CHP2_FGFR3_5	FGFR3	18	72.8	14	15.7
CHP2_FGFR3_3	FGFR3	14	66	9	10.1