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. 2015 Sep 14;10(9):e0136699. doi: 10.1371/journal.pone.0136699

Fig 4. SP-D treatment results in a decrease in infectivity.

Fig 4

ACCM-2 or L-929 passaged C. burnetii were stained with CSFE and treated overnight with SP-D or the equivalent volume of buffer alone (PBS). MH-S cells were infected at MOI 100 for 4 or 8 hours and fixed. MH-S nuclei were stained with Hoescht, the number of infected cells and bacteria was quantified via confocal microscopy, and the infectivity index was calculated (bound and internalized C. burnetii). Data are displayed as mean ± standard deviation of infectivity from one representative of 5 experiments, n = 3. Student t-tests compare SP-D to PBS treated groups. *, p<0.05, ***, p<0.0005.