Table 1. Numbers of animals used for each experiment.
| Experiment | Ctr | 1 day | 2 days | 3 days | 5 days | 7 days | 2 wks | 5 wks | 10 wks | 17 wks | 6 mths | 1 yr | Total |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| NF180 IHC | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 5 | 5 | 5 | 5 | 2 | 36 |
| 4 wks-1 dye | 5 wks-2 dyes | Total | |||||||||||
| Hemi-TX | 4 | 2 | 6 | ||||||||||
| 2 wks | 4 wks | 9 wks | Total | ||||||||||
| Ctr-MO | 8 | 8 | 3 | 19 | |||||||||
| NF180-MO | 8 | 8 | 3 | 19 |
Times in the top row are in days (d), weeks (w) or year (yr) after spinal cord transection (TX). Ctrl, control; Tot, total; IHC, immunohistochemistry; MO, morpholino antisense oligonucleotide. In the 4-week experiment, a single dye (DAF 488, green) was applied to a complete TX 4 weeks after, and 1 cm caudal to an unlabeled hemisection (hemi-TX) to look for axon collaterals crossing the midline. In the 5-week experiment, one dye (DMTR, red) was applied to a hemisection, and then 4 weeks later, a second dye (DAF 488, green) was applied to a complete TX 1 cm caudally.