Fig 2. CBS and CSE expression and activity in human T24 cells.

(A) CBS and CSE protein expression in T24 cells and human urothelium tissue. Loading in the gel lanes was controlled by detection of GAPDH protein. (B) T24 cell homogenates generate detectable amount of H₂S in basal (vehicle) or in stimulated (L-cysteine) conditions (*p<0.05 vs vehicle). (C) 8-Br-cGMP incubation causes a significant increase in H₂S production at 15 or 30 min (°°p<0.001 and °p<0.05 at 15 and 30 min, respectively). Incubation with the PKG inhibitor, KT5823, abrogates the H₂S production (^###p<0.001). d-cAMP incubation for 5, 15 and 30 min in the presence or in the absence of the PKA inhibitor, KT5720, did not affect H₂S production. Data are calculated as nanomoles per milligram of protein per min and expressed as mean±SE of six different experiments.