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. 2015 Sep 14;10(9):e0137994. doi: 10.1371/journal.pone.0137994

Fig 2. Effects of post translational modifications on the N-terminal domain of MscL determined by in vivo screens.

Fig 2

The viability of the osmotically fragile strain MJF455 expressing MscL cysteine substituted mutants from residues S2 to D18 (insert), was measured after an osmotic down-shock. The graphs show the differences in viability between non-treated and post-transnationally modified channels with (A) the hydrophobic MTS reagents MTSBn (blue), decyl-MTS (red) or 4HB-MTS (green) or (B) the negatively charged MTSES- (blue) and positively charged MTSET+ (red). The red grid line indicates a ± 50% change that was used as a threshold for further studies.