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. 2015 Sep 14;10(9):e0137994. doi: 10.1371/journal.pone.0137994

Fig 11. Functional changes by substitutions in the C-terminal domain of MscL, determined by in vivo and patch clamp experiments.

Fig 11

(A) The location of the residues showing changes in viability upon post-translational modifications is highlighted in the closed structure of E. coli MscL. From left to right: a pentameric MscL is shown in a side view where the approximate location of the membrane marked, a single subunit and a close up of the region with the labeled residues followed by a cytoplasmic view. (B) Viability MJF 367 (mscL -) cells shock in the presence of the indicated MTS reagent is shown for each individual MscL mutant. C. The changes in the pressure threshold required to gate R104C, K105C and K106C MscL caused by MTS reagents is graphed as the ratio between before and after modification of the same patch. The red line indicates no change.